Journal Article

Behaviour of a Population of Partially Duplicated Mitochondrial DNA Molecules in Cell Culture: Segregation, Maintenance and Recombination Dependent Upon Nuclear Background

I. J. Holt, D. R. Dunbar and H. T. Jacobs

in Human Molecular Genetics

Volume 6, issue 8, pages 1251-1260
Published in print August 1997 | ISSN: 0964-6906
Published online August 1997 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/6.8.1251
Behaviour of a Population of Partially Duplicated Mitochondrial DNA Molecules in Cell Culture: Segregation, Maintenance and Recombination Dependent Upon Nuclear Background

Show Summary Details

Preview

We have studied the dynamics of mitochondrial DNA maintenance and segregation in human cells using serial cybrid transfer of partially duplicated mito-chondrial DNA, from a mitochondrial myopathy patient, to two distinct recipient cell types. The results indicate two radically different outcomes dependent upon nuclear background. In one case (lung carcinoma) there is systematic loss of the partial duplication by an implied recombinational mechanism. In another nuclear background (osteosarcoma) the duplicated molecules can survive, having only a marginal effect on mitochondrial respiratory function. Moreover, in the osteosarcoma nuclear background further disturbances of mtDNA maintenance frequently follow from cybrid transfer. These are progressive, catastrophic loss of mtDNA and further rearrangement to generate partially triplicated molecules. The results imply differential expression of nuclear genes regulating mtDNA copy number, replication and recombination in different human cell types.

Journal Article.  7789 words.  Illustrated.

Subjects: Genetics and Genomics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.