Journal Article

Sequence Comparison of Human and Yeast Telomeres Identifies Structurally Distinct Subtelomeric Domains

J. Flint, G. P. Bates, K. Clark, A. Dorman, D. Willingham, B. A. Roe, G. Micklem, D. R. Higgs and E. J. Louis

in Human Molecular Genetics

Volume 6, issue 8, pages 1305-1314
Published in print August 1997 | ISSN: 0964-6906
Published online August 1997 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/6.8.1305
Sequence Comparison of Human and Yeast Telomeres Identifies Structurally Distinct Subtelomeric Domains

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We have sequenced and compared DNA from the ends of three human chromosomes: 4p, 16p and 22q. In all cases the pro-terminal regions are subdivided by degenerate (TTAGGG)n repeats into distal and proximal sub-domains with entirely different patterns of homology to other chromosome ends. The distal regions contain numerous, short (<2 kb) segments of interrupted homology to many other human telomeric regions. The proximal regions show much longer (10–40 kb) uninterrupted homology to a few chromosome ends. A comparison of all yeast subtelomeric regions indicates that they too are subdivided by degenerate TTAGGG repeats into distal and proximal sub-domains with similarly different patterns of identity to other non-homologous chromosome ends. Sequence comparisons indicate that the distal and proximal sub-domains do not interact with each other and that they interact quite differently with the corresponding regions on other, non-homologous, chromosomes. These findings suggest that the degenerate TTAGGG repeats identify a previously unrecognized, evolutionarily conserved boundary between remarkably different subtelomeric domains.

Journal Article.  6429 words.  Illustrated.

Subjects: Genetics and Genomics

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