Journal Article

Human Mini-Chromosomes in Mouse Embryonal Stem Cells

M. H. Shen, J. Yang, M.-L. Loupart, A. Smith and W. Brown

in Human Molecular Genetics

Volume 6, issue 8, pages 1375-1382
Published in print August 1997 | ISSN: 0964-6906
Published online August 1997 | e-ISSN: 1460-2083 | DOI:
Human Mini-Chromosomes in Mouse Embryonal Stem Cells

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We have introduced human mini-chromosomes of 4 Mb and ∼15 Mb in size into mouse embryonal stem cells. Although these human mini-chromosomes are stable in hamster and chicken cells, they re-arrange or segregate aberrantly in the embryonal stem cells and are rapidly lost in the absence of selection. However, one of the mini-chromosomes re-arranged, acquired mouse centromeric sequences and was then stably maintained for at least 60 population doublings in culture. This mini-chromosome, which is 4 Mb in size, is a candidate for a mouse germ line chromosome vector.

Journal Article.  5824 words.  Illustrated.

Subjects: Genetics and Genomics

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