Journal Article

The Preliminary Transcript Map of a Human Skeletal Muscle

Alberto Pallavicini, Rosanna Zimbello, Natascia Tiso, Teresa Muraro, Luca Rampoldi, Stefania Bortoluzzi, Giorgio Valle, Gerolamo Lanfranchi and Gian A. Danieli

in Human Molecular Genetics

Volume 6, issue 9, pages 1445-1450
Published in print September 1997 | ISSN: 0964-6906
Published online September 1997 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/6.9.1445
The Preliminary Transcript Map of a Human Skeletal Muscle

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By sequencing 11 405 individual expressed sequence tags (ESTs) from a cDNA library of a human skeletal muscle, we identified 1945 individual transcripts, 725 of which showed no correspondence with known human genes. We report here the chromosomal localization of 267 of these, obtained by radiation hybrid (RH) mapping. The map position of additional 242 ESTs from the same library, corresponding to known human genes, is also reported. The resulting information provides a preliminary genomic transcriptional profile of a human muscle. Several genes occur in clusters on different chromosomes. Moreover, chromosomes 17, 19, 21 and X appear to be significantly rich in muscle ESTs. By analysing several collections of ESTs from different tissues, we observed significant deviations in the distribution of ESTs by chromosome in fetal heart, adult brain and adult retina, supporting the hypothesis that a non-random localization of genes expressed in specific tissues might not be uncommon. The selective concentration of expressed genes in some chromosomes and in specific chromosomal subregions in a given tissue might reflect the existence of batteries of genes under the same control mechanisms, regulating tissue-specific gene expression.

Journal Article.  3861 words.  Illustrated.

Subjects: Genetics and Genomics

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