Journal Article

Nuclear Localization of SYT, SSX and the Synovial Sarcoma-Associated SYT-SSX Fusion Proteins

Nuno R. dos Santos, Diederik R. H. de Bruijn, Matthé Balemans, Bert Janssen, Fátima Gärtner, José Manuel Lopes, Bertie de Leeuw and Ad Geurts van Kessel

in Human Molecular Genetics

Volume 6, issue 9, pages 1549-1558
Published in print September 1997 | ISSN: 0964-6906
Published online September 1997 | e-ISSN: 1460-2083 | DOI:
Nuclear Localization of SYT, SSX and the Synovial Sarcoma-Associated SYT-SSX Fusion Proteins

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Synovial sarcoma is characterized by a prevalent chromosomal translocation, t(X;18)(p11;q11). As a result of this translocation the SYT gene on chromosome 18 fuses to either the SSX1 or the SSX2 gene on the X chromosome. In this study, we generated polyclonal antibodies against the SYT and SSX2 proteins. These antibodies specifically detected both these proteins and the SYT-SSX fusion proteins in transfected COS-1 cell extracts. Indirect immunofluorescence analysis of COS-1 cells expressing tagged or untagged SYT, SSX2, SYT-SSX1 or SYT-SSX2 indicated that all these proteins are localized in the nucleus, excluding the nucleoli. The SSX2 protein exhibited a diffuse staining pattern whereas both the SYT and SYT-SSX proteins appeared in several nuclear dots. Similar nuclear dots were also detected in primary synovial sarcoma cells growing in a short-term in vitro culture. Double immunofluorescence in conjunction with confocal laser-scanning microscopy revealed that the SYT and SYT-SSX nuclear dots do not co-localize with known nuclear structures as e.g. coiled bodies, SC35 interchromatin granules or PML bodies. The similar nuclear localization patterns of SYT and SYT-SSX suggest that the SYT-SSX fusion proteins are directed to SYT-associated nuclear domains where an abnormal function may be exerted.

Journal Article.  7182 words.  Illustrated.

Subjects: Genetics and Genomics

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