Journal Article

Characterization of Genes Encoding Translation Initiation Factor eIF-2γ in Mouse and Human: Sex Chromosome Localization, Escape from X-Inactivation and Evolution

Ingrid E. Ehrmann, Pamela S. Ellis, Sophie Mazeyrat, Sarah Duthie, Neil Brockdorff, Marie G. Mattei, Marc A. Gavin, Nabeel A. Affara, Graeme M. Brown, Elizabeth Simpson, Michael J. Mitchell and Diane M. Scott

in Human Molecular Genetics

Volume 7, issue 11, pages 1725-1737
Published in print October 1998 | ISSN: 0964-6906
Published online October 1998 | e-ISSN: 1460-2083 | DOI: https://dx.doi.org/10.1093/hmg/7.11.1725
Characterization of Genes Encoding Translation Initiation Factor eIF-2γ in Mouse and Human: Sex Chromosome Localization, Escape from X-Inactivation and Evolution

Show Summary Details

Preview

The ΔSxrb interval of the mouse Y chromosome is critical for spermatogenesis and expression of the male-specific minor transplantation antigen H-Y. Several genes have been mapped to this interval and each has a homologue on the X chromosome. Four, Zfyl, Zfy2, Ubely and Dffry, are expressed specifically in the testis and their X homologues are not transcribed from the inactive X chromosome. A further two, Smcy and Uty, are ubiquitously expressed and their X homologues escape X-inactivation. Here we report the identification of another gene from this region of the mouse Y chromosome. It encodes the highly conserved eukaryotic translation initiation factor eIF-2γ. In the mouse this gene is ubiquitously expressed, has an X chromosome homologue which maps close to Dmd and escapes X-inactivation. The coding regions of the X and Y genes show 86% nucleotide identity and encode putative products with 98% amino acid identity. In humans, the eIF-2γ structural gene is located on the X chromosome at Xp21 and this also escapes X-inactivation. However, there is no evidence of a Y copy of this gene in humans. We have identified autosomal retroposons of eIF-2γ in both humans and mice and an additional retroposon on the X chromosome in some mouse strains. Ark blot analysis of eutherian and metatherian genomic DNA indicates that X-Y homologues are present in all species tested except simian primates and kangaroo and that retroposons are common to a wide range of mammals. These results shed light on the evolution of X-Y homologous genes.

Journal Article.  10121 words.  Illustrated.

Subjects: Genetics and Genomics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.