Journal Article

<i>mdx</i> Muscle Pathology is Independent of nNOS Perturbation

Rachelle H. Crosbie, Volker Straub, Hye-Young Yun, Jane C. Lee, Jill A. Rafael, Jeffrey S. Chamberlain, Valina L. Dawson, Ted M. Dawson and Kevin P. Campbell

in Human Molecular Genetics

Volume 7, issue 5, pages 823-829
Published in print May 1998 | ISSN: 0964-6906
Published online May 1998 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/7.5.823
mdx Muscle Pathology is Independent of nNOS Perturbation

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In skeletal muscle, neuronal nitric oxide synthase (nNOS) is anchored to the sarcolemma via the dystrophin-glycoprotein complex. When dystrophin is absent, as in Duchenne muscular dystrophy patients and in mdx mice, nNOS is mislocalized to the interior of the muscle fiber where it continues to produce nitric oxide. This has led to the hypothesis that free radical toxicity from mislocalized nNOS may contribute to mdx muscle pathology. To test this hypothesis directly, we generated mice devoid of both nNOS and dystrophin. Overall, the nNOS-dystrophin null mice maintained the dystrophic characteristics of mdx mice. We evaluated the mice for several features of the dystrophic phenotype, including membrane damage and muscle morphology. Removal of nNOS did not alter the extent of sarcolemma damage, which is a hallmark of the dystrophic phenotype. Furthermore, muscle from nNOS-dystrophin null mice maintain the histological features of mdx pathology. Our results demonstrate that relocalization of nNOS to the cytosol does not contribute significantly to mdx pathogenesis.

Journal Article.  4575 words.  Illustrated.

Subjects: Genetics and Genomics

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