Journal Article

<i>Xist</i> RNA Exhibits a Banded Localization on the Inactive X Chromosome and Is Excluded from Autosomal Material <i>in Cis</i>

Sarah M. Duthie, Tatyana B. Nesterova, Emma J. Formstone, Ann M. Keohane, Bryan M. Turner, Suren M. Zakian and Neil Brockdorff

in Human Molecular Genetics

Volume 8, issue 2, pages 195-204
Published in print February 1999 | ISSN: 0964-6906
Published online February 1999 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/8.2.195
Xist RNA Exhibits a Banded Localization on the Inactive X Chromosome and Is Excluded from Autosomal Material in Cis

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The propagation of X chromosome inactivation is thought to be mediated by the cis-limited spreading of the nonprotein coding Xist transcript. In this report we have investigated the localization of Xist RNA on rodent metaphase chromosomes. We show that Xist RNA exhibits a banded pattern on the inactive X and is excluded from regions of constitutive heterochromatin. The banding pattern suggests a preferential association with gene-rich, G-light regions. Analysis of X:autosome rearrangements revealed that restricted propagation of X inactivation into cis-linked autosomal material is reflected by a corresponding limited spread of Xist RNA. We discuss these results in the context of models for the function of Xist RNA in the propagation of X inactivation.

Journal Article.  7727 words.  Illustrated.

Subjects: Genetics and Genomics

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