Journal Article

Circular Dystrophin RNAs Consisting of Exons That Were Skipped by Alternative Splicing

Agus Surono, Yasuhiro Takeshima, Tri Wibawa, Makoto Ikezawa, Ikuya Nonaka and Masafumi Matsuo

in Human Molecular Genetics

Volume 8, issue 3, pages 493-500
Published in print March 1999 | ISSN: 0964-6906
Published online March 1999 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/8.3.493
Circular Dystrophin RNAs Consisting of Exons That Were Skipped by Alternative Splicing

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Exon skipping by alternative splicing and circular RNA formation are proposed to be interrelated events. Since multiple patterns of alternative splicing have been demonstrated in both the 5′ and 3′ regions of the dystrophin gene, the dystrophin transcript in skeletal muscle cells provides a model system in which this idea is tested. Nine circular RNAs that were expected to result from known exon skipping patterns in the 5′ region of this gene were in fact identified, but three other circular RNAs expected to result from other known exon skipping reactions in this region could not be detected. The identification of two unexpected circular RNAs led to the discovery of two novel alternative splicing reactions. One circular RNA originating in the 3′ region of the gene was identified but it lacked one small sized exon compared with the expected exon structure. Circular RNAs from the 5′ region of the dystrophin gene could not be detected in Duchenne muscular dystrophy patients who have deletions of one or more exons in this segment of the gene, even though alternative splicing products were identified. These results showed that circular RNA formation is not necessarily linked to exon skipping and suggest that an undetermined factor regulates circular RNA formation.

Journal Article.  5814 words.  Illustrated.

Subjects: Genetics and Genomics

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