Journal Article

Inner Ear and Kidney Anomalies Caused by IAP Insertion in an Intron of the <i>Eya1</i> Gene in a Mouse Model of BOR Syndrome

Kenneth R. Johnson, Susan A. Cook, Lawrence C. Erway, Angela N. Matthews, L. Phillip Sanford, Nancy E. Paradies and Rick A. Friedman

in Human Molecular Genetics

Volume 8, issue 4, pages 645-653
Published in print April 1999 | ISSN: 0964-6906
Published online April 1999 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/8.4.645
Inner Ear and Kidney Anomalies Caused by IAP Insertion in an Intron of the Eya1 Gene in a Mouse Model of BOR Syndrome

Show Summary Details

Preview

A spontaneous mutation causing deafness and circling behavior was discovered in a C3H/HeJ colony of mice at the Jackson Laboratory. Pathological analysis of mutant mice revealed gross morphological abnormalities of the inner ear, and also dysmorphic or missing kidneys. The deafness and abnormal behavior were shown to be inherited as an autosomal recessive trait and mapped to mouse chromosome 1 near the position of the Eya1 gene. The human homolog of this gene, EYA1, has been shown to underly branchio-oto-renal (BOR) syndrome, an autosomal dominant disorder characterized by hearing loss with associated branchial and renal anomalies. Molecular analysis of the Eya1 gene in mutant mice revealed the insertion of an intracisternal A particle (IAP) element in intron 7. The presence of the IAP insertion was associated with reduced expression of the normal Eya1 message and formation of additional aberrant transcripts. The hypomorphic nature of the mutation may explain its recessive inheritance, if protein levels in homozygotes, but not heterozygotes, are below a critical threshold needed for normal developmental function. The new mouse mutation is designated Eya1bor to denote its similarity to human BOR syndrome, and will provide a valuable model for studying mutant gene expression and etiology.

Journal Article.  5079 words.  Illustrated.

Subjects: Genetics and Genomics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.