Journal Article

T-STAR/ÉTOILE: A Novel Relative of SAM68 That Interacts with an RNA-Binding Protein Implicated in Spermatogenesis

J. P. Venables, C. Vernet, S. L. Chew, D. J. Elliott, R. B. Cowmeadow, J. Wu, H. J. Cooke, K. Artzt and I. C. Eperon

in Human Molecular Genetics

Volume 8, issue 6, pages 959-969
Published in print June 1999 | ISSN: 0964-6906
Published online June 1999 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/8.6.959
T-STAR/ÉTOILE: A Novel Relative of SAM68 That Interacts with an RNA-Binding Protein Implicated in Spermatogenesis

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RBM is an RNA-binding protein encoded on the Y chromosome in mammals and is expressed only in the nuclei of male germ cells. Genetic evidence from infertile men implicates it in spermatogenesis, but its function is unknown. Of a number of potential partners for RBM identified by a yeast two-hybrid screen with testis cDNA, the most frequent isolates encoded a novel RNA-binding protein, termed T-STAR, that is closely related to SAM68, an Src-associated protein of unknown function. The mouse homologue was also cloned and designated étoile. It mapped to chromosome 15, while T-STAR mapped to the syntenic region on human chromosome 8. T-STAR/étoileis expressed primarily in the testis; in rat germ cells, the expression of both T-STAR/étoile and SAM68 is regulated during meiosis. Transfection of T-STAR/étoile fused with green fluorescent protein into HeLa cells caused an accumulation of protein in a novel compartment of the nucleus, adjacent to the nucleolus but distinct from the perinucleolar compartment. RBM and other hnRNP G family members are candidate downstream targets for regulation by T-STAR/ÉTOILE and SAM68.

Journal Article.  8388 words.  Illustrated.

Subjects: Genetics and Genomics

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