Journal Article

Human mini-chromosomes with minimal centromeres

J.W. Yang, C. Pendon, J. Yang, N. Haywood, A. Chand and W.R.A. Brown

in Human Molecular Genetics

Volume 9, issue 12, pages 1891-1902
Published in print July 2000 | ISSN: 0964-6906
Published online July 2000 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/9.12.1891
Human mini-chromosomes with minimal centromeres

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We have introduced a 6.5 Mb human mini-chromosome with a complex centromere structure into DT40 cells and have used sequence targeting and telomere-directed chromosome breakage to dissect the sequence requirements for centromere function. These experiments proved that a vertebrate centromere with two blocks of functional alphoid DNA separated by 2.5 Mb can exist as a stable structure in some but not all vertebrate cells. Further experiments indicated that recovery of chromosomes with less than 100 kb of alphoid DNA is very inefficient, suggesting that a functional centromere requires a minimum of 100 kb of alphoid DNA. Mini-chromosomes with minimal centromeres segregate accurately in some but not all vertebrate cells and should be useful for the detection of sequence-specific factors required for vertebrate centromere maintenance.

Journal Article.  8159 words.  Illustrated.

Subjects: Genetics and Genomics

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