Journal Article

Transduction of 3′-flanking sequences is common in L1 retrotransposition

John L. Goodier, Eric M. Ostertag and Haig H. Kazazian Jr

in Human Molecular Genetics

Volume 9, issue 4, pages 653-657
Published in print March 2000 | ISSN: 0964-6906
Published online March 2000 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/9.4.653
Transduction of 3′-flanking sequences is common in L1 retrotransposition

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Active LINE-1 (L1) elements possess the ability to transduce non-L1 DNA flanking their 3′ ends to new genomic locations. Occasionally, the 3′ end processing machinery may bypass the L1 polyadenylation signal and instead utilize a second downstream polyadeny‐ lation site. To determine the frequency of L1-mediated transduction in the human genome, we selected 66 previously uncharacterized L1 sequences from the GenBank database. Fifteen (23%) of these L1s had transposed flanking DNA with an average transduction length of 207 nucleotides. Since there are ∼400 000 L1 elements, we estimate that insertion of transduced sequences alone may have enlarged the diploid human genome as much as 19 Mb or 0.6%. We also examined 24 full-length mouse L1s and found two long transduced sequences. Thus, L1 retrotransposition in vivo com‐ monly transduces sequence flanking the 3′ end of the element.

Journal Article.  2981 words.  Illustrated.

Subjects: Genetics and Genomics

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