Journal Article

SNPs in the promoter of a B cell-specific antisense transcript, <i>SAS-ZFAT</i>, determine susceptibility to autoimmune thyroid disease

Senji Shirasawa, Haruhito Harada, Koichi Furugaki, Takashi Akamizu, Naofumi Ishikawa, Kunihiko Ito, Koichi Ito, Hajime Tamai, Kanji Kuma, Sumihisa Kubota, Hitomi Hiratani, Tomoko Tsuchiya, Iwai Baba, Mayuko Ishikawa, Masao Tanaka, Kenji Sakai, Masayuki Aoki, Ken Yamamoto and Takehiko Sasazuki

in Human Molecular Genetics

Volume 13, issue 19, pages 2221-2231
Published in print October 2004 | ISSN: 0964-6906
Published online August 2004 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/ddh245
SNPs in the promoter of a B cell-specific antisense transcript, SAS-ZFAT, determine susceptibility to autoimmune thyroid disease

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Autoimmune thyroid disease (AITD) is caused by an immune response to self-thyroid antigens and has a significant genetic component. Antisense RNA transcripts have been implicated in gene regulation. Here we have identified a novel zinc-finger gene, designated ZFAT (zinc-finger gene in AITD susceptibility region), as one of the susceptibility genes in 8q23–q24 through an initial association analysis using the probands in the previous linkage analysis and a subsequent association analysis of the samples from a total of 515 affected individuals and 526 controls. The T allele of the single-nucleotide polymorphism (SNP), Ex9b-SNP10 located in the intron 9 of ZFAT, is associated with increased risk for AITD (dominant model: odds ratio=1.7, P=0.000091). The Ex9b-SNP10 falls into the 3′-UTR of truncated-ZFAT (TR-ZFAT) and the promoter region of the small antisense transcript of ZFAT (SAS-ZFAT). In peripheral blood lymphocytes, SAS-ZFAT is exclusively expressed in CD19+ B cells and expression levels of SAS-ZFAT and TR-ZFAT seemed to correlate with the Ex9b-SNP10-T-associated ZFAT-allele, inversely and positively, respectively. The Ex9b-SNP10 is critically involved in the regulation of SAS-ZFAT expression in vitro and this expression results in a decreased expression of TR-ZFAT. These results suggested that the SNP-associated ZFAT-allele plays a critical role in B cell function by affecting the expression level of TR-ZFAT through regulating SAS-ZFAT expression and that this novel regulatory mechanism of SNPs might be involved in controlling susceptibility or resistance to human disease.

Journal Article.  6480 words.  Illustrated.

Subjects: Genetics and Genomics

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