Journal Article

Parkin enhances mitochondrial biogenesis in proliferating cells

Yukiko Kuroda, Takao Mitsui, Makoto Kunishige, Masayuki Shono, Masashi Akaike, Hiroyuki Azuma and Toshio Matsumoto

in Human Molecular Genetics

Volume 15, issue 6, pages 883-895
Published in print March 2006 | ISSN: 0964-6906
Published online January 2006 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/ddl006
Parkin enhances mitochondrial biogenesis in proliferating cells

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We describe a novel function of parkin, a RING protein, which is elaborately involved in mitochondrial biogenesis. Parkin was located within the mitochondrial organelle of proliferating cells. Anti-proliferative treatments released parkin from mitochondria to cytosol. Results of pharmacological treatments indicate that parkin was released from mitochondria when permeability transition pore was opened. The extra-mitochondrial localization was also observed in differentiated cells. In proliferating cells, transcription and replication of mitochondrial DNA was enhanced by parkin overexpression and attenuated by parkin suppression with siRNA. Parkin was associated with mitochondrial transcription factor A (TFAM) and enhanced TFAM-mediated mitochondrial transcription. These results indicate that parkin is involved in the regulation of mitochondrial transcription/replication other than the ubiquitin-mediated protein degradation system in proliferating cells.

Journal Article.  7265 words.  Illustrated.

Subjects: Genetics and Genomics

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