Journal Article

MPP1 links the Usher protein network and the Crumbs protein complex in the retina

Ilse Gosens, Erwin van Wijk, Ferry F.J. Kersten, Elmar Krieger, Bert van der Zwaag, Tina Märker, Stef J.F. Letteboer, Simone Dusseljee, Theo Peters, Henk A. Spierenburg, Ingrid M. Punte, Uwe Wolfrum, Frans P.M. Cremers, Hannie Kremer and Ronald Roepman

in Human Molecular Genetics

Volume 16, issue 16, pages 1993-2003
Published in print August 2007 | ISSN: 0964-6906
Published online June 2007 | e-ISSN: 1460-2083 | DOI:
MPP1 links the Usher protein network and the Crumbs protein complex in the retina

Show Summary Details


The highly ordered distribution of neurons is an essential feature of a functional mammalian retina. Disruptions in the apico-basal polarity complexes at the outer limiting membrane (OLM) of the retina are associated with retinal patterning defects in vertebrates. We have analyzed the binding repertoire of MPP5/Pals1, a key member of the apico-basal Crumbs polarity complex, that has functionally conserved counterparts in zebrafish (nagie oko) and Drosophila (Stardust). We show that MPP5 interacts with its MAGUK family member MPP1/p55 at the OLM. Mechanistically, this interaction involves heterodimerization of both MAGUK modules in a directional fashion. MPP1 expression in the retina throughout development resembles the expression of whirlin, a multi-PDZ scaffold protein and an important organizer in the Usher protein network. We demonstrate that both proteins interact strongly by both a classical PDZ domain-to-PDZ binding motif (PBM) mechanism, and a mechanism involving internal epitopes. MPP1 and whirlin colocalize in the retina at the OLM, at the outer synaptic layer and at the basal bodies and the ciliary axoneme. In view of the known roles of the Crumbs and Usher protein networks, our findings suggest a novel link of the core developmental processes of actin polymerization and establishment/maintenance of apico-basal cell polarity through MPP1. These processes, essential in neural development and patterning of the retina, may be disrupted in eye disorders that are associated with defects in these protein networks.

Journal Article.  6459 words.  Illustrated.

Subjects: Genetics and Genomics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.