Journal Article

Activation of β-catenin signaling by Rspo1 controls differentiation of the mammalian ovary

Anne-Amandine Chassot, Fariba Ranc, Elodie P. Gregoire, Hermien L. Roepers-Gajadien, Makoto M. Taketo, Giovanna Camerino, Dirk G. de Rooij, Andreas Schedl and Marie-Christine Chaboissier

in Human Molecular Genetics

Volume 17, issue 9, pages 1264-1277
Published in print May 2008 | ISSN: 0964-6906
Published online February 2008 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/ddn016
Activation of β-catenin signaling by Rspo1 controls differentiation of the mammalian ovary

Show Summary Details

Preview

The sex of an individual is determined by the fate of the gonad. While the expression of Sry and Sox9 is sufficient to induce male development, we here show that female differentiation requires activation of the canonical β-catenin signaling pathway. β-catenin activation is controlled by Rspo1 in XX gonads and Rspo1 knockout mice show masculinized gonads. Molecular analyses demonstrate an absence of female-specific activation of Wnt4 and as a consequence XY-like vascularization and steroidogenesis. Moreover, germ cells of XX knockout embryos show changes in cellular adhesions and a failure to enter XX specific meiosis. Sex cords develop around birth, when Sox9 becomes strongly activated. Thus, a balance between Sox9 and β-catenin activation determines the fate of the gonad, with Rspo1 acting as a crucial regulator of canonical β-catenin signaling required for female development.

Journal Article.  6926 words.  Illustrated.

Subjects: Genetics and Genomics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.