Journal Article

FANCM of the Fanconi anemia core complex is required for both monoubiquitination and DNA repair

Yutong Xue, Yongjiang Li, Rong Guo, Chen Ling and Weidong Wang

in Human Molecular Genetics

Volume 17, issue 11, pages 1641-1652
Published in print June 2008 | ISSN: 0964-6906
Published online February 2008 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/ddn054
FANCM of the Fanconi anemia core complex is required for both monoubiquitination and DNA repair

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In response to DNA damage, the Fanconi anemia (FA) core complex functions as a signaling machine for monoubiquitination of FANCD2 and FANCI. It remains unclear whether this complex can also participate in subsequent DNA repair. We have shown previously that the FANCM constituent of the complex contains a highly conserved helicase domain and an associated ATP-dependent DNA translocase activity. Here we show that FANCM also possesses an ATP-independent binding activity and an ATP-dependent bi-directional branch-point translocation activity on a synthetic four-way junction DNA, which mimics intermediates generated during homologous recombination or at stalled replication forks. Using an siRNA-based complementation system, we found that the ATP-dependent activities of FANCM are required for cellular resistance to a DNA-crosslinking drug, mitomycin C, but not for the monoubiquitination of FANCD2 and FANCI. In contrast, monoubiquitination requires the entire helicase domain of FANCM, which has both ATP dependent and independent activities. These data are consistent with participation of FANCM and its associated FA core complex in the FA pathway at both signaling through monoubiquitination and the ensuing DNA repair.

Journal Article.  6967 words.  Illustrated.

Subjects: Genetics and Genomics

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