Journal Article

Stabilization of β-catenin in XY gonads causes male-to-female sex-reversal

Danielle M. Maatouk, Leo DiNapoli, Ashley Alvers, Keith L. Parker, Makoto M. Taketo and Blanche Capel

in Human Molecular Genetics

Volume 17, issue 19, pages 2949-2955
Published in print October 2008 | ISSN: 0964-6906
Published online July 2008 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/ddn193
Stabilization of β-catenin in XY gonads causes male-to-female sex-reversal

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During mammalian sex determination, expression of the Y-linked gene Sry shifts the bipotential gonad toward a testicular fate by upregulating a feed-forward loop between FGF9 and SOX9 to establish SOX9 expression in somatic cells. We previously proposed that these signals are mutually antagonistic with counteracting signals in XX gonads and that a shift in the balance of these factors leads to either male or female development. Evidence in mice and humans suggests that the male pathway is opposed by the expression of two signals, WNT4 and R-SPONDIN-1 (RSPO1), that promote the ovarian fate and block testis development. Both of these ligands can activate the canonical Wnt signaling pathway. Duplication of the distal portion of chromosome 1p, which includes both WNT4 and RSPO1, overrides the male program and causes male-to-female sex reversal in XY patients. To determine whether activation of β-catenin is sufficient to block the testis pathway, we have ectopically expressed a stabilized form of β-catenin in the somatic cells of XY gonads. Our results show that activation of β-catenin in otherwise normal XY mice effectively disrupts the male program and results in male-to-female sex-reversal. The identification of β-catenin as a key pro-ovarian and anti-testis signaling molecule will further our understanding of the mechanisms controlling sex determination and the molecular mechanisms that lead to sex-reversal.

Journal Article.  4680 words.  Illustrated.

Subjects: Genetics and Genomics

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