Journal Article

The cell adhesion molecule nectin-1 is critical for normal enamel formation in mice

Martin J. Barron, Steven J. Brookes, Clare E. Draper, David Garrod, Jennifer Kirkham, Roger C. Shore and Michael J. Dixon

in Human Molecular Genetics

Volume 17, issue 22, pages 3509-3520
Published in print November 2008 | ISSN: 0964-6906
Published online August 2008 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/ddn243
The cell adhesion molecule nectin-1 is critical for normal enamel formation in mice

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Nectin-1 is a member of a sub-family of immunoglobulin-like adhesion molecules and a component of adherens junctions. In the current study, we have shown that mice lacking nectin-1 exhibit defective enamel formation in their incisor teeth. Although the incisors of nectin-1-null mice were hypomineralized, the protein composition of the enamel matrix was unaltered. While strong immunostaining for nectin-1 was observed at the interface between the maturation-stage ameloblasts and the underlying cells of the stratum intermedium (SI), its absence in nectin-1-null mice correlated with separation of the cell layers at this interface. Numerous, large desmosomes were present at this interface in wild-type mice; however, where adhesion persisted in the mutant mice, the desmosomes were smaller and less numerous. Nectins have been shown to regulate tight junction formation; however, this is the first report showing that they may also participate in the regulation of desmosome assembly. Importantly, our results show that integrity of the SI–ameloblast interface is essential for normal enamel mineralization.

Journal Article.  6981 words.  Illustrated.

Subjects: Genetics and Genomics

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