Journal Article

Association of the thyroid stimulating hormone receptor gene (<i>TSHR</i>) with Graves’ disease

Oliver J. Brand, Jeffrey C. Barrett, Matthew J. Simmonds, Paul R. Newby, Christopher J. McCabe, Christopher K. Bruce, Boris Kysela, Jackie D. Carr-Smith, Thomas Brix, Penny J. Hunt, Wilmar M. Wiersinga, Laszlo Hegedüs, John Connell, John A.H. Wass, Jayne A. Franklyn, Anthony P. Weetman, Joanne M. Heward and Stephen C.L. Gough

in Human Molecular Genetics

Volume 18, issue 9, pages 1704-1713
Published in print May 2009 | ISSN: 0964-6906
Published online February 2009 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/ddp087
Association of the thyroid stimulating hormone receptor gene (TSHR) with Graves’ disease

Show Summary Details

Preview

Graves’ disease (GD) is a common autoimmune disease (AID) that shares many of its susceptibility loci with other AIDs. The thyroid stimulating hormone receptor (TSHR) represents the primary autoantigen in GD, in which autoantibodies bind to the receptor and mimic its ligand, thyroid stimulating hormone, causing the characteristic clinical phenotype. Although early studies investigating the TSHR and GD proved inconclusive, more recently we provided convincing evidence for association of the TSHR region with disease. In the current study, we investigated a combined panel of 98 SNPs, including 70 tag SNPs, across an extended 800 kb region of the TSHR to refine association in a cohort of 768 GD subjects and 768 matched controls. In total, 28 SNPs revealed association with GD (P < 0.05), with strongest SNP associations at rs179247 (χ2 = 32.45, P = 8.90 × 10−8, OR = 1.53, 95% CI = 1.32–1.78) and rs12101255 (χ2 = 30.91, P = 1.95 × 10−7, OR = 1.55, 95% CI = 1.33–1.81), both located in intron 1 of the TSHR. Association of the most associated SNP, rs179247, was replicated in 303 GD families (P = 7.8 × 10−4). In addition, we provide preliminary evidence that the disease-associated genotypes of rs179247 (AA) and rs12101255 (TT) show reduced mRNA expression ratios of flTSHR relative to two alternate TSHR mRNA splice variants.

Journal Article.  5999 words.  Illustrated.

Subjects: Genetics and Genomics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.