Journal Article

Epigenomic profiling indicates a role for DNA methylation in early postnatal liver development

Robert A. Waterland, Richard Kellermayer, Marie-Therese Rached, Nina Tatevian, Marcus V. Gomes, Jiexin Zhang, Li Zhang, Abrita Chakravarty, Wei Zhu, Eleonora Laritsky, Wenjuan Zhang, Xiaodan Wang and Lanlan Shen

in Human Molecular Genetics

Volume 18, issue 16, pages 3026-3038
Published in print August 2009 | ISSN: 0964-6906
Published online May 2009 | e-ISSN: 1460-2083 | DOI:
Epigenomic profiling indicates a role for DNA methylation in early postnatal liver development

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The question of whether DNA methylation contributes to the stabilization of gene expression patterns in differentiated mammalian tissues remains controversial. Using genome-wide methylation profiling, we screened 3757 gene promoters for changes in methylation during postnatal liver development to test the hypothesis that developmental changes in methylation and expression are temporally correlated. We identified 31 genes that gained methylation and 111 that lost methylation from embryonic day 17.5 to postnatal day 21. Promoters undergoing methylation changes in postnatal liver tended not to be associated with CpG islands. At most genes studied, developmental changes in promoter methylation were associated with expression changes, suggesting both that transcriptional inactivity attracts de novo methylation, and that transcriptional activity can override DNA methylation and successively induce developmental hypomethylation. These in vivo data clearly indicate a role for DNA methylation in mammalian differentiation, and provide the novel insight that critical windows in mammalian developmental epigenetics extend well beyond early embryonic development.

Journal Article.  7203 words.  Illustrated.

Subjects: Genetics and Genomics

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