Journal Article

<i>Fancm</i>-deficient mice reveal unique features of Fanconi anemia complementation group M

Sietske T. Bakker, Henri J. van de Vrugt, Martin A. Rooimans, Anneke B. Oostra, Jurgen Steltenpool, Elly Delzenne-Goette, Anja van der Wal, Martin van der Valk, Hans Joenje, Hein te Riele and Johan P. de Winter

in Human Molecular Genetics

Volume 18, issue 18, pages 3484-3495
Published in print September 2009 | ISSN: 0964-6906
Published online June 2009 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/ddp297
Fancm-deficient mice reveal unique features of Fanconi anemia complementation group M

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The Fanconi anemia (FA) core complex member FANCM remodels synthetic replication forks and recombination intermediates. Thus far, only one FA patient with FANCM mutations has been described, but the relevance of these mutations for the FA phenotype is uncertain. To provide further experimental access to the FA-M complementation group we have generated Fancm-deficient mice by deleting exon 2. FANCM deficiency caused hypogonadism in mice and hypersensitivity to cross-linking agents in mouse embryonic fibroblasts (MEFs), thus phenocopying other FA mouse models. However, FancmΔ2/Δ2 mice also showed unique features atypical for FA mice, including underrepresentation of female FancmΔ2/Δ2 mice and decreased overall and tumor-free survival. This increased cancer incidence may be correlated to the role of FANCM in the suppression of spontaneous sister chromatid exchanges as observed in MEFs. In addition, FANCM appeared to have a stimulatory rather than essential role in FANCD2 monoubiquitination. The FA-M mouse model presented here suggests that FANCM functions both inside and outside the FA core complex to maintain genome stability and to prevent tumorigenesis.

Journal Article.  7167 words.  Illustrated.

Subjects: Genetics and Genomics

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