Journal Article

Germline competency of parthenogenetic embryonic stem cells from immature oocytes of adult mouse ovary

Zhong Liu, Zhe Hu, Xinghua Pan, Minshu Li, Taiwo A. Togun, David Tuck, Mattia Pelizzola, Junjiu Huang, Xiaoying Ye, Yu Yin, Mengyuan Liu, Chao Li, Zhisheng Chen, Fang Wang, Lingjun Zhou, Lingyi Chen, David L. Keefe and Lin Liu

in Human Molecular Genetics

Volume 20, issue 7, pages 1339-1352
Published in print April 2011 | ISSN: 0964-6906
Published online January 2011 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/ddr016
Germline competency of parthenogenetic embryonic stem cells from immature oocytes of adult mouse ovary

Show Summary Details

Preview

Parthenogenetic embryonic stem cells (pESCs) have been generated in several mammalian species from parthenogenetic embryos that would otherwise die around mid-gestation. However, previous reports suggest that pESCs derived from in vivo ovulated (IVO) mature oocytes show limited pluripotency, as evidenced by low chimera production, high tissue preference and especially deficiency in germline competence, a critical test for genetic integrity and pluripotency of ESCs. Here, we report efficient generation of germline-competent pESC lines (named as IVM pESCs) from parthenogenetic embryos developed from immature oocytes of adult mouse ovaries following in vitro maturation (IVM) and artificial activation. In contrast, pESCs derived from IVO oocytes show defective germline competence, consistent with previous reports. Further, IVM pESCs resemble more ESCs from fertilized embryos (fESCs) than do IVO pESCs on genome-wide DNA methylation and global protein profiles. In addition, IVM pESCs express higher levels of Blimp1, Lin28 and Stella, relative to fESCs, and in their embryoid bodies following differentiation. This may indicate differences in differentiation potentially to the germline. The mechanisms for acquisition of pluripotency and germline competency of IVM pESCs from immature oocytes remain to be determined.

Journal Article.  7674 words.  Illustrated.

Subjects: Genetics and Genomics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.