Journal Article

Dual transcriptional activator and repressor roles of TBX20 regulate adult cardiac structure and function

Noboru J. Sakabe, Ivy Aneas, Tao Shen, Leila Shokri, Soo-Young Park, Martha L. Bulyk, Sylvia M. Evans and Marcelo A. Nobrega

in Human Molecular Genetics

Volume 21, issue 10, pages 2194-2204
Published in print May 2012 | ISSN: 0964-6906
Published online February 2012 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/dds034
Dual transcriptional activator and repressor roles of TBX20 regulate adult cardiac structure and function

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The ongoing requirement in adult heart for transcription factors with key roles in cardiac development is not well understood. We recently demonstrated that TBX20, a transcriptional regulator required for cardiac development, has key roles in the maintenance of functional and structural phenotypes in adult mouse heart. Conditional ablation of Tbx20 in adult cardiomyocytes leads to a rapid onset and progression of heart failure, with prominent conduction and contractility phenotypes that lead to death. Here we describe a more comprehensive molecular characterization of the functions of TBX20 in adult mouse heart. Coupling genome-wide chromatin immunoprecipitation and transcriptome analyses (RNA-Seq), we identified a subset of genes that change expression in Tbx20 adult cardiomyocyte-specific knockout hearts which are direct downstream targets of TBX20. This analysis revealed a dual role for TBX20 as both a transcriptional activator and a repressor, and that each of these functions regulates genes with very specialized and distinct molecular roles. We also show how TBX20 binds to its targets genome-wide in a context-dependent manner, using various cohorts of co-factors to either promote or repress distinct genetic programs within adult heart. Our integrative approach has uncovered several novel aspects of TBX20 and T-box protein function within adult heart.

Sequencing data accession number (http://www.ncbi.nlm.nih.gov/geo): GSE30943.

Journal Article.  7411 words.  Illustrated.

Subjects: Genetics and Genomics

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