Journal Article

Polymorphisms in the human cysteine-rich secretory protein 2 (<i>CRISP2</i>) gene in Australian men

D. Jamsai, A. Reilly, S.J. Smith, G.M. Gibbs, H.W.G. Baker, R.I. McLachlan, D.M. de Kretser and M.K. O'Bryan

in Human Reproduction

Published on behalf of European Society of Human Reproduction and Embryology

Volume 23, issue 9, pages 2151-2159
Published in print September 2008 | ISSN: 0268-1161
Published online June 2008 | e-ISSN: 1460-2350 | DOI: https://dx.doi.org/10.1093/humrep/den191
Polymorphisms in the human cysteine-rich secretory protein 2 (CRISP2) gene in Australian men

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BACKGROUND

Cysteine-rich secretory protein 2 (CRISP2) is localized to the human sperm acrosome and tail. It can regulate ryanodine receptors Ca2+ gating and binds to mitogen-activated protein kinase kinase kinase 11 in the acrosome and gametogenetin 1 (GGN1) in the tail.

METHODS AND RESULTS

In order to test the hypothesis that CRISP2 variations contribute to male infertility, we screened coding and flanking intronic regions in 92 infertile men with asthenozoo- and/or teratozoospermia and 176 control men using denaturing HPLC and sequencing. There were 21 polymorphisms identified, including 13 unreported variations. Three SNPs resulted in amino acid substitutions: L59V, M176I and C196R. All were only present in a heterozygous state and found in fertile men. However, the C196R polymorphism was of particular interest as it resulted in the loss of a strictly conserved cysteine involved in intramolecular disulphide bonding. Screening of an additional 637 infertile men identified 23 heterozygous C196R men to give an overall frequency of 3.6%, compared with 3.4% in control men. The functional significance of the C196R polymorphism was defined using a yeast two-hybrid assay. The C196R substitution resulted in the loss of CRISP2–GGN1 binding.

CONCLUSIONS

Although none of the many polymorphisms identified herein showed a significant association with male infertility, functional studies suggested that the C196R polymorphism may compromise CRISP2 function.

Keywords: CRISP2; TPX1; GGN; male infertility; single-nucleotide polymorphism

Journal Article.  6459 words.  Illustrated.

Subjects: Reproductive Medicine

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