Journal Article

Phenotypes of the ovarian follicular basal lamina predict developmental competence of oocytes

Helen F. Irving-Rodgers, Stephanie Morris, Rachael A. Collett, Teija T. Peura, Margaret Davy, Jeremy G. Thompson, Helen D. Mason and Raymond J. Rodgers

in Human Reproduction

Published on behalf of European Society of Human Reproduction and Embryology

Volume 24, issue 4, pages 936-944
Published in print April 2009 | ISSN: 0268-1161
Published online December 2008 | e-ISSN: 1460-2350 | DOI: https://dx.doi.org/10.1093/humrep/den447
Phenotypes of the ovarian follicular basal lamina predict developmental competence of oocytes

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BACKGROUND

The ovarian follicular basal lamina underlies the epithelial membrana granulosa and maintains the avascular intra-follicular compartment. Additional layers of basal lamina occur in a number of pathologies, including pili annulati and diabetes. We previously found additional layers of follicular basal lamina in a significant percentage of healthy bovine follicles. We wished to determine if this phenomenon existed in humans, and if it was related to oocyte function in the bovine.

METHODS AND RESULTS

We examined follicles from human ovaries (n = 18) by electron microscopy and found that many follicles had additional layers of basal lamina. Oocytes (n = 222) from bovine follicles with normal or unusual basal laminas were isolated and their ability to undergo in vitro maturation, fertilization and culture to blastocyst was compared. Healthy bovine follicles with a single layer of basal lamina had oocytes with significantly (P < 0.01) greater developmental competence than healthy follicles with additional layers of follicular basal lamina (65% versus 28%).

CONCLUSIONS

These findings provide direct evidence that the phenotype of the follicular basal lamina is related to oocyte competence.

Keywords: ovary; basal lamina; oocyte competence; in vitro production

Journal Article.  4764 words.  Illustrated.

Subjects: Reproductive Medicine

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