Journal Article

Identification of <i>Girella punctata</i> and <i>G. leonina</i> by PCR-RFLP analysis

Shiro Itoi, Takashi Saito, Mai Shimojo, Sayaka Washio and Haruo Sugita

in ICES Journal of Marine Science

Published on behalf of ICES/CIEM

Volume 64, issue 2, pages 328-331
Published in print March 2007 | ISSN: 1054-3139
Published online November 2006 | e-ISSN: 1095-9289 | DOI: http://dx.doi.org/10.1093/icesjms/fsl023
Identification of Girella punctata and G. leonina by PCR-RFLP analysis

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Itoi, S., Saito, T., Shimojo, M., Washio, S., and Sugita, H. 2007. Identification of Girella punctata and G. leonina by PCR-RFLP analysis. – ICES Journal of Marine Science, 64: 328–331.

Two Girella species, Girella punctata and G. leonina, are sympatric sister species with an extensive overlap in their respective distributions on shallow rocky reefs from Hong Kong to the south of the Japanese Islands. Juveniles of the two species cannot be discriminated easily on the basis of external characters. In this study, after morphological identification of the species, sequencing analysis was carried out for the partial 16S ribosomal RNA gene and for the D-loop region in mitochondrial DNA. A total of 109 specimens was examined. Restriction site mapping of the sequences suggested that the electrophoretic patterns of polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) analysis of the gene products would produce a species-specific banding pattern. Subsequently, the PCR-RFLP analysis showed that the method was as effective for separating the two morphologically similar species of the genus Girella as the sequencing analysis.

Keywords: D-loop; Girella leonina; Girella punctata; mtDNA; PCR–RFLP; 16S rRNA

Journal Article.  2363 words.  Illustrated.

Subjects: Environmental Science ; Marine and Estuarine Biology

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