Journal Article

Plasma and Mucosal Immunoglobulin M, Immunoglobulin A, and Immunoglobulin G Responses to the <i>Vibrio cholerae</i> O1 Protein Immunome in Adults With Cholera in Bangladesh

Richelle C. Charles, Rie Nakajima, Li Liang, Al Jasinskas, Amanda Berger, Daniel T. Leung, Meagan Kelly, Peng Xu, Pavol Kováč, Samantha R. Giffen, James D. Harbison, Fahima Chowdhury, Ashraful I. Khan, Stephen B. Calderwood, Taufiqur Rahman Bhuiyan, Jason B. Harris, Philip L. Felgner, Firdausi Qadri and Edward T. Ryan

in The Journal of Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 216, issue 1, pages 125-134
Published in print July 2017 | ISSN: 0022-1899
Published online May 2017 | e-ISSN: 1537-6613 | DOI: http://dx.doi.org/10.1093/infdis/jix253
Plasma and Mucosal Immunoglobulin M, Immunoglobulin A, and Immunoglobulin G Responses to the Vibrio cholerae O1 Protein Immunome in Adults With Cholera in Bangladesh

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Abstract

Background.

Cholera is a severe dehydrating illness of humans caused by toxigenic strains of Vibrio cholerae O1 or O139. Identification of immunogenic V. cholerae antigens could lead to a better understanding of protective immunity in human cholera.

Methods.

We probed microarrays containing 3652 V. cholerae antigens with plasma and antibody-in-lymphocyte supernatant (ALS, a surrogate marker of mucosal immune responses) from patients with severe cholera caused by V. cholerae O1 in Bangladesh and age-, sex-, and ABO-matched Bangladeshi controls. We validated a subset of identified antigens using enzyme-linked immunosorbent assay.

Results.

Overall, we identified 608 immunoreactive V. cholerae antigens in our screening, 59 of which had higher immunoreactivity in convalescent compared with acute-stage or healthy control samples (34 in plasma, 39 in mucosal ALS; 13 in both sample sets). Identified antigens included cholera toxin B and A subunits, V. cholerae O–specific polysaccharide and lipopolysaccharide, toxin coregulated pilus A, sialidase, hemolysin A, flagellins (FlaB, FlaC, and FlaD), phosphoenolpyruvate-protein phosphotransferase, and diaminobutyrate–2-oxoglutarate aminotransferase.

Conclusions.

This study is the first antibody profiling of the mucosal and systemic antibody responses to the nearly complete V. cholerae O1 protein immunome; it has identified antigens that may aid in the development of an improved cholera vaccine.

Keywords: cholera; Vibrio; cholerae; immunogenic; OSP; sialidase; HlyA.

Journal Article.  5802 words.  Illustrated.

Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

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