Journal Article

CD4<sup>+</sup>Thy1<sup>−</sup> thymocytes with a T<sub>h</sub>-type 2 cytokine response

Douglas M. Cerasoli, Garnett Kelsoe and Marcella Sarzotti

in International Immunology

Published on behalf of Japanese Society for Immunology

Volume 13, issue 1, pages 75-83
Published in print February 2001 | ISSN: 0953-8178
Published online February 2001 | e-ISSN: 1460-2377 | DOI: http://dx.doi.org/10.1093/intimm/13.1.75
CD4+Thy1− thymocytes with a Th-type 2 cytokine response

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We have identified a small subset of CD3+, CD4+, CD8 thymocytes that do not express Thy1 (CD90). This Thy1 subset represents 1–3.7% of the total number of thymocytes in a naive mouse. CD4+Thy1 thymocytes express high levels of CD3, intermediate to high levels of heat-stable antigen (HSA), and low levels of CD25, CD45RB, CD69, CD44 and CD62L. They produce high titers of IL-4 and no IFN-γ upon stimulation in vitro, a response characteristic of Th2 cells. In the thymi of mice infected neonatally with a high dose of the retrovirus Cas-Br-E MuLV, the frequency of CD4+Thy1 cells increased ~10-fold. High-dose virus infection resulted in decreased HSA and increased CD44 expression on CD4+Thy1 cells relative to cells from naive mice. CD4+Thy1 cells from high-dose infected mice also secreted IL-4 and not IFN-γ upon in vitro stimulation. We previously reported that infection of newborn mice with a high dose of murine retrovirus results in the induction of a non-protective anti-viral Th2 T cell response; CD4+Thy1 thymocytes with a Th2-like cytokine profile may play a role in determining the cytokine bias of this anti-viral response.

Keywords: Cas-Br-E MuLV; CD4+ cells; newborn mice; Th2; Thy1– thymocytes; thymic atrophy; 7AAD 7-aminoactinomyosin D; APC antigen-presenting cell; Cas Cas-Br-E MuLV; Con A concanavalin A; HSA heat-stable antigen; MuLV murine leukemia virus; PEC peritoneal exudate cell; p.i. post-infection

Journal Article.  5595 words.  Illustrated.

Subjects: Immunology ; Biological Sciences

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