Journal Article

Thymic expression of peripheral tissue antigens in humans: a remarkable variability among individuals

Hiroshi Takase, Cheng-Rong Yu, Rashid M. Mahdi, Daniel C. Douek, Gregory B. DiRusso, Frank M. Midgley, Rajpreet Dogra, Gloria Allende, Eliot Rosenkranz, Alberto Pugliese, Charles E. Egwuagu and Igal Gery

in International Immunology

Published on behalf of Japanese Society for Immunology

Volume 17, issue 8, pages 1131-1140
Published in print August 2005 | ISSN: 0953-8178
Published online July 2005 | e-ISSN: 1460-2377 | DOI: http://dx.doi.org/10.1093/intimm/dxh275
Thymic expression of peripheral tissue antigens in humans: a remarkable variability among individuals

More Like This

Show all results sharing these subjects:

  • Immunology
  • Biological Sciences

GO

Show Summary Details

Preview

The majority of maturing T lymphocytes that recognize self-antigens is eliminated in the thymus upon exposure to their target antigens. This physiological process of negative selection requires that tissue-specific antigens be expressed by thymic cells, a phenomenon that has been well studied in experimental animals. Here, we have examined the expression in human thymi of four retinal antigens, that are capable of inducing autoimmune ocular disease retinal S-antigen (S-Ag), recoverin, RPE65 and inter-photoreceptor retinoid-binding protein (IRBP)], as well as four melanocyte-specific antigens, two of which are used as targets for melanoma immunotherapy [gp100, melanoma antigen recognized by T cells 1, tyrosinase-related protein (TRP)-1 and TRP-2]. Using reverse transcription (RT)–PCR, we found that all thymic samples from the 18 donors expressed mRNA transcripts of most or all the eight tested tissue antigens. Yet, the expression of the transcripts varied remarkably among the individual thymic samples. In addition, S-Ag, RPE65 and IRBP were detected by immunostaining in rare cells in sections of human thymi by antibodies against these proteins. Quantitative real-time RT–PCR analysis revealed that the retinal antigen transcripts in the human thymus are present at trace levels, that are lower by approximately five orders of magnitude than those in the retina. Our observations thus support the notions that thymic expression is a common feature for all tissue-specific antigens and that the levels of expression play a role in determining the susceptibility to autoimmunity against these molecules.

Keywords: autoimmunity; retinal antigen; susceptibility to disease; tolerance; tumor immunity; IRBP    inter-photoreceptor retinoid-binding protein; MART    melanoma antigen recognized by T cells; NEI    National Eye Institute; NIH    National Institutes of Health; PLP    proteolipid protein; RT    reverse transcription; S-Ag    retinal S-antigen; TRITC    tetramethylrhodamine isothiocyanate; TRP    tyrosinase-related protein

Journal Article.  5592 words.  Illustrated.

Subjects: Immunology ; Biological Sciences

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.