Journal Article

Interplay between T<sub>h</sub>1 and T<sub>h</sub>17 effector T-cell pathways in the pathogenesis of spontaneous colitis and colon cancer in the Gαi2-deficient mouse

Yu-Yuan Götlind, Maria Fritsch Fredin, Ashok Kumar Kumawat, Hilja Strid, Roger Willén, Ignacio Rangel, Paul W. Bland and Elisabeth Hultgren Hörnquist

in International Immunology

Volume 25, issue 1, pages 35-44
Published in print January 2013 | ISSN: 0953-8178
Published online September 2012 | e-ISSN: 1460-2377 | DOI:
Interplay between Th1 and Th17 effector T-cell pathways in the pathogenesis of spontaneous colitis and colon cancer in the Gαi2-deficient mouse

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Gαi2-deficient mice spontaneously develop colitis. Using xMAP technology and RT–PCR, we investigated cytokine/chemokine profiles during histologically defined phases of disease: (i) no/mild, (ii) moderate, (iii) severe colitis without dysplasia/cancer and (iv) severe colitis with dysplasia/cancer, compared with age-matched wild-type (WT) littermates. Colonic dysplasia was observed in 4/11 mice and cancer in 1/11 mice with severe colitis. The histology correlated with progressive increases in colon weight/cm and spleen weight, and decreased thymus weight, all more advanced in mice with dysplasia/cancer. IL-1β, IL-6, IL-12p40, IL-17, TNF-α, CCL2 and CXCL1 protein levels in colons, but not small intestines increased with colitis progression and were significantly increased in mice with moderate and severe colitis compared with WT mice, irrespective of the absence/presence of dysplasia/cancer. CCL5 did not change during colitis progression. Colonic IL-17 transcription increased 40- to 70-fold in all stages of colitis, whereas IFN-γ mRNA was gradually up-regulated 12- to 55-fold with colitis progression, and further to 62-fold in mice with dysplasia/cancer. IL-27 mRNA increased 4- to 15-fold during the course of colitis, and colonic IL-21 transcription increased 3-fold in mice with severe colitis, both irrespective of the absence/presence of dysplasia/cancer. FoxP3 transcription was significantly enhanced (3.5-fold) in mice with moderate and severe colitis, but not in mice with dysplasia/cancer, compared with WT mice. Constrained correspondence analysis demonstrated an association between increased protein levels of TNF-α, CCL2, IL-1β, IL-6 and CXCL1 and dysplasia/cancer. In conclusion, colonic responses are dominated by a mixed Th1/Th17 phenotype, with increasing Th1 cytokine transcription with progression of colitis in Gαi2–/– mice.

Keywords: chemokines; constrained correspondence analysis; dysplasia; inflammatory bowel disease; real-time RT–PCR

Journal Article.  5881 words.  Illustrated.

Subjects: Immunology ; Biological Sciences

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