Journal Article

Nuclear transferred embryonic stem cells for analysis of B1 B-lymphocyte development

Mitsuyo Takase, Ryuji Iida, Mikako Maruya, Asako Sakaue-Sawano, Atsushi Miyawaki, Teruhiko Wakayama, Satoshi Nishigami, Sidonia Fagarasan and Osami Kanagawa

in International Immunology

Volume 25, issue 3, pages 145-156
Published in print March 2013 | ISSN: 0953-8178
Published online October 2012 | e-ISSN: 1460-2377 | DOI: http://dx.doi.org/10.1093/intimm/dxs095
Nuclear transferred embryonic stem cells for analysis of B1 B-lymphocyte development

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The transfer of nuclei of fully differentiated cells into enucleated oocytes is a well-recognized method for the generation of embryonic stem (ES) cells. Here, we demonstrate that nuclear transferred ES (NT-ES) cells can be established with high efficiency using innate-like B lymphocytes as donor cells. We established two mouse lines carrying rearranged immunoglobulin heavy and light chains using NT-ES cells containing nuclei from peritoneal cavity B1 cells. Analysis of B1 clone lines revealed that the B1-cell generation critically depends on the interaction between antigen (possibly self-antigen) and surface immunoglobulin, while the B1-cell maintenance requires the peritoneal environment. The B1-cell expansion takes place in spleen, and is held in check by competitor B2 cells. The results indicate that the NT-ES method could replace the transgenic or knock-in mouse approaches currently used to study the biology of cells that undergo somatic rearrangements of their antigen receptor genes.

Keywords: antigen receptor; B1 cells; monoclonal mice; nuclear transfer

Journal Article.  7427 words.  Illustrated.

Subjects: Immunology ; Biological Sciences

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