Journal Article

Partial characterization of a transposon containing the <i>tet</i>(A) determinant in a clinical isolate of <i>Acinetobacter baumannii</i>

Anna Ribera, Ignasi Roca, Joaquim Ruiz, Isidre Gibert and Jordi Vila

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 52, issue 3, pages 477-480
Published in print September 2003 | ISSN: 0305-7453
Published online September 2003 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkg344
Partial characterization of a transposon containing the tet(A) determinant in a clinical isolate of Acinetobacter baumannii

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A genomic library from one clinical isolate of Acinetobacter baumannii was obtained to find genes responsible for tetracycline resistance. Escherichia coli DH5α-MDR transformants, selected on Mueller–Hinton agar supplemented with tetracycline 18 mg/L, were thoroughly characterized. In one clone, with an insert of 7070 bp, it was found that the resistance to tetracycline was mediated by the tet(A) gene (1200 bp) which encodes a tetracycline efflux pump. This gene was recovered together with tetR(A) (651 bp), the tet(A) repressor. Moreover, the partial sequence (2008 bp) of a transposase gene, tnpA, and 1316 bp corresponding to an IS, similar to that described in one strain of Salmonella typhi (IS4321), were found. In this A. baumannii clinical isolate, the tet(A) gene is located in a transposon. The structure of this transposon is similar to that of Tn1721, with the tet(A), tetR(A) and the regions between these genes, being closely related to those of Tn1721. The data indicate horizontal transfer of tetracycline resistance genes between A. baumannii and other genera sharing the same ecological niches.

Keywords: Keywords: tetracycline resistance, Acinetobacter baumannii

Journal Article.  2399 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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