Journal Article

Comparison of the Sensititre YeastOne colorimetric antifungal panel and Etest with the NCCLS M38-A method to determine the activity of amphotericin B and itraconazole against clinical isolates of <i>Aspergillus</i> spp.

Estrella Martín-Mazuelos, Javier Pemán, Anastasio Valverde, Mónica Chaves, M. Carmen Serrano and Emilia Cantón

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 52, issue 3, pages 365-370
Published in print September 2003 | ISSN: 0305-7453
Published online September 2003 | e-ISSN: 1460-2091 | DOI: https://dx.doi.org/10.1093/jac/dkg384
Comparison of the Sensititre YeastOne colorimetric antifungal panel and Etest with the NCCLS M38-A method to determine the activity of amphotericin B and itraconazole against clinical isolates  of Aspergillus spp.

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Objective: The evaluation of the Sensititre YeastOne and Etest methods for determining susceptibility to amphotericin B and itraconazole by comparing the MICs obtained by these methods at different times of reading with those of the M38-A broth microdilution method.

Methods: Sixty-three clinical isolates of Aspergillus spp. (23 Aspergillus flavus, 24 Aspergillus fumigatus, nine Aspergillus niger, three Aspergillus glaucus, two Aspergillus terreus and two Aspergillus flavipes) were assayed. Two itraconazole-resistant strains (NCPF7100 and 7099) were also included.

Results: Itraconazole MICs for the two resistant strains were >4 mg/L by the three methods. The overall agreement (±2 log2) between M38-A (48 h) and colorimetric (48 h) method was 93.4% for amphotericin B and 90.2% for itraconazole. By the Etest, the best agreement with M38-A was obtained when readings were made at 24 h: 88.5% for amphotericin B and 67.2% for itraconazole. Etest MICs were higher for all species except A. niger.

Conclusions: The colorimetric method appears to be a suitable alternative procedure for antifungal susceptibility testing of Aspergillus spp. and is able to detect resistance to itraconazole. The range of MICs for amphotericin B by Etest is wider and for some strains is >16 mg/L, suggesting that this method could be useful for detecting resistant strains as occur in yeasts.

Keywords: Keywords: Aspergillus spp., susceptibility testing, MIC, Sensititre YeastOne

Journal Article.  4341 words. 

Subjects: Medical Oncology ; Critical Care

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