Journal Article

Diversity of TEM-52 extended-spectrum β-lactamase-producing non-typhoidal <i>Salmonella</i> isolates in Korea

Kyungwon Lee, Dongeun Yong, Jong Hwa Yum, Ho Hoon Kim and Yunsop Chong

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 52, issue 3, pages 493-496
Published in print September 2003 | ISSN: 0305-7453
Published online September 2003 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkg385
Diversity of TEM-52 extended-spectrum β-lactamase-producing non-typhoidal Salmonella isolates in Korea

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Objectives: Extended-spectrum β-lactamase (ESBL)-producing non-typhoidal Salmonella (NTS) isolates in Korea were characterized.

Patients and methods: Five isolates of ESBL-producing NTS were isolated from stool specimens of three infants and two adults with diarrhoea. Two infants acquired the infection in the community, and three other infections were hospital acquired.

Results: The isolates were one each of serovars Saintpaul, Stanley and Agona, and two Enteritidis. Cell sonicates of the isolates hydrolysed cefotaxime more efficiently than ceftazidime, and had β-lactamase bands of approximate isoelectric points 6.0 and 7.4. Sequencing revealed that the β-lactamases were TEM-52 and an OXA type. The blaOXA gene was located on a class 1 integron. Cefotaxime resistance, associated with TEM-52, was transferred by conjugation. Identical pulsed-field gel electrophoresis patterns of XbaI-digested genomic DNA were observed in initially β-lactam-susceptible serovar Agona isolates and subsequent ESBL-producing isolates from an infant, and in two isolates of serovar Enteritidis from two different patients.

Conclusions: This study suggests that TEM-52-producing NTS is spreading both clonally and horizontally in Korea.

Keywords: Keywords: enteritis, class 1 integron, OXA-type β-lactamase

Journal Article.  2080 words. 

Subjects: Medical Oncology ; Critical Care

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