Journal Article

Development of a microdilution method to evaluate <i>Mycobacterium tuberculosis</i> drug susceptibility

Elena Banfi, Giuditta Scialino and Carlo Monti-Bragadin

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 52, issue 5, pages 796-800
Published in print November 2003 | ISSN: 0305-7453
Published online November 2003 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkg439
Development of a microdilution method to evaluate Mycobacterium tuberculosis drug susceptibility

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A new rapid microdilution method, employing the dye resazurin as an indicator of mycobacterial growth, was developed to evaluate drug susceptibility of Mycobacterium tuberculosis reference strain H37Rv and of 13 M. tuberculosis susceptible or multidrug-resistant clinical strains. Different growth conditions were evaluated. The MICs of isoniazid, rifampicin, streptomycin and ethambutol were determined by the Microdilution Resazurin Assay (MRA) and the results compared with those obtained by the agar proportion method; complete agreement was always obtained. MRA resulted in a rapid, reliable, simple and inexpensive coloured method suitable for testing the susceptibility of M. tuberculosis clinical strains to first-line drugs; its employment in evaluating new antibacterial molecules is also suggested.

Keywords: Keywords: M. tuberculosis, resazurin, MIC evaluation, microdilution assay, first-line antitubercular drugs

Journal Article.  3424 words. 

Subjects: Medical Oncology ; Critical Care

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