Journal Article

SHV-34: an extended-spectrum β-lactamase encoded by an epidemic plasmid

John Heritage, Philip A. Chambers, Caroline Tyndall and E. Stephen Buescher

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 52, issue 6, pages 1015-1017
Published in print December 2003 | ISSN: 0305-7453
Published online December 2003 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkh017
SHV-34: an extended-spectrum β-lactamase encoded by an  epidemic plasmid

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Objectives: To elucidate the causes for treatment failure in children given extended-spectrum cephalosporins.

Methods: During April 1998–March 2000, 18 isolates of members of the family Enterobacteriaceae, fulfilling microbiological criteria for carriage of extended-spectrum β-lactamases (ESBLs) and carrying blaSHV, were isolated from paediatric inpatients. The collection was subjected to a retrospective molecular analysis.

Results: Three species were represented in the collection: Citrobacter koseri (one isolate), Escherichia coli (one isolate) and Klebsiella pneumoniae (16 isolates). A common plasmid was found in these bacteria, as judged by restriction endonuclease digestion. This was able to transfer an ESBL phenotype from donors to a laboratory strain of E. coli. Nucleotide sequence analysis revealed that this phenotype was associated with a new variant in blaSHV encoding SHV-34.

Conclusions: Analysis reveals the presence of an epidemic plasmid in this collection of bacteria. This carries a gene encoding the SHV-34 ESBL, described for the first time in this report. Nucleotide sequence analysis shows that there is a mutation from A→G affecting the codon at amino acid position 64 (GAA→GGA), changing the glutamic acid typically seen in this position to glycine.

Keywords: Keywords: ESBLs, Enterobacteriaceae, SHV β-lactamase

Journal Article.  1779 words. 

Subjects: Medical Oncology ; Critical Care

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