Journal Article

BRO β-lactamase alleles, antibiotic resistance and a test of the BRO-1 selective replacement hypothesis in <i>Moraxella catarrhalis</i>

F. Levy and E. S. Walker

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 53, issue 2, pages 371-374
Published in print February 2004 | ISSN: 0305-7453
Published online February 2004 | e-ISSN: 1460-2091 | DOI: https://dx.doi.org/10.1093/jac/dkh063
BRO β-lactamase alleles, antibiotic resistance and a test of the BRO-1 selective replacement hypothesis in Moraxella catarrhalis

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Objectives: The hypothesis that BRO-1 selectively replaced the BRO-2 isoform of the Moraxella catarrhalis BRO β-lactamase was tested by examining the temporal distribution, antibiotic resistance and epidemiological characteristics of isolates from a long-term collection at a single locale.

Methods: A rapid, one-step PCR assay conducted on 354 isolates spanning 1984–1994 distinguished bro alleles in over 97% of the β-lactamase-producing isolates. Probes of dot blots were used to distinguish PCR failure from non-β-lactamase-mediated penicillin resistance.

Results: BRO-2 isolates comprised 0–10% of the population per year with no evidence of a decline over time. All β-lactamase producers exceeded the clinical threshold for penicillin resistance. Bimodality of penicillin MICs for β-lactamase producers was caused by variation within BRO-1 rather than differences between BRO-1 and BRO-2. Non-β-lactamase factors also confer resistance to penicillin and may contribute to the BRO-1 bimodality. The 13 BRO-2 isolates were associated with diverse genotypes within which there was evidence of epidemiologically linked clusters. The exclusive association of BRO-2 with four unrelated genotypes suggested maintenance of BRO-2 by recurrent mutation or horizontal exchange.

Conclusions: The relative rarity of BRO-2 throughout the study, the absence of a declining temporal trend, and genetic diversity within BRO-2 all failed to support the hypothesis that BRO-2 was more common in the past and has been selectively replaced by BRO-1.

Keywords: Keywords: disease transmission, molecular epidemiology, selection

Journal Article.  2129 words. 

Subjects: Medical Oncology ; Critical Care

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