Journal Article

A comparative study of the disc diffusion method with the broth microdilution and Etest methods for voriconazole susceptibility testing of <i>Aspergillus</i> spp.

M. C. Serrano, M. Ramírez, D. Morilla, A. Valverde, M. Chávez, A. Espinel-Ingroff, R. Claro, A. Fernández, C. Almeida and E. Martín-Mazuelos

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 53, issue 5, pages 739-742
Published in print May 2004 | ISSN: 0305-7453
Published online May 2004 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkh172
A comparative study of the disc diffusion method with the broth microdilution and Etest methods for voriconazole susceptibility testing of Aspergillus spp.

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Objective: The activity of voriconazole against Aspergillus spp. (n = 77) was tested by the Etest, disc diffusion and the NCCLS M38-A methods.

Methods: Four Rhizomucor spp. isolates were included to study the suitability of the three susceptibility testing methods to detect isolates resistant to voriconazole. The disc diffusion method performed on Mueller–Hinton agar (Difco) supplemented with 2% glucose and Methylene Blue (0.5 mg/L) gave zone diameters with very clear border edges.

Results: The disc diffusion method showed excellent correlation with the Etest and the NCCLS methods.

Conclusion: On the basis of our results, it appears that the disc diffusion test is a useful method for testing the activity of voriconazole against Aspergillus spp.

Keywords: Keywords: antifungals, susceptibility tests

Journal Article.  2293 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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