Journal Article

Class 1 integrons in various <i>Salmonella enterica</i> serovars isolated from animals and identification of genomic island SGI1 in <i>Salmonella enterica</i> var. Meleagridis

Paul Ebner, Kimberly Garner and Alan Mathew

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 53, issue 6, pages 1004-1009
Published in print June 2004 | ISSN: 0305-7453
Published online June 2004 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkh192
Class 1 integrons in various Salmonella enterica serovars isolated from animals and identification of genomic island SGI1 in  Salmonella enterica var. Meleagridis

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Objectives: To determine the prevalence of integron-mediated antibiotic resistance in a diverse sample set of Salmonella enterica isolated from animals.

Materials and methods: Multiplex PCR was used to detect class 1 integron gene sequences, and integron gene cassettes were identified by PCR mapping. Susceptibility to 18 antibiotics or antibiotic combinations commonly used in either human or veterinary medicine was measured using a microdilution method, and statistical comparisons of the frequency of resistance between groups were made using Fisher’s two-sided probability test. Genotypic comparisons of isolates were made following pulsed-field gel electrophoresis of genomic DNA.

Results: Thirty-two (30.8%) of 104 isolates contained class 1 integron sequences. Integron-positive isolates represented 15 different S. enterica serovars, were obtained from nine different animal species and had a higher frequency of non-integron-mediated antibiotic resistance (P < 0.05) compared with integron-negative isolates. One non-Typhimurium isolate (S. enterica Meleagridis) contained an SGI1 genomic island, including the antibiotic resistance gene cluster.

Conclusions: These data demonstrate that integron-mediated antibiotic resistance is common among diverse Salmonella serovars, many of them rare. In addition, SGI1 is not limited to Salmonella enterica Typhimurium DT104 or other commonly isolated serovars.

Keywords: Keywords: antibiotic resistance, integrons, Salmonella, SGI1

Journal Article.  3547 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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