Journal Article

Molecular characterization of class 1 integrons from Irish thermophilic <i>Campylobacter</i> spp.

Fiona O’Halloran, Brigid Lucey, Bartley Cryan, Tom Buckley and Séamus Fanning

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 53, issue 6, pages 952-957
Published in print June 2004 | ISSN: 0305-7453
Published online June 2004 | e-ISSN: 1460-2091 | DOI:
Molecular characterization of class 1 integrons from Irish thermophilic Campylobacter spp.

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  • Medical Oncology
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Objectives: In this study a large random collection (n = 378) of Irish thermophilic Campylobacter isolates were investigated for the presence of integrons, genetic elements associated with the dissemination of antimicrobial resistance.

Methods: Purified genomic DNA from each isolate was analysed by PCR for the presence of class 1 integrons. Four gene cassette-associated amplicons were completely characterized.

Results: Sixty-two of the isolates possessed a complete class 1 integron with a recombined gene cassette located within a 1.0 kb amplicon containing an aadA2 gene. This cassette was present in both Campylobacter jejuni and Campylobacter coli isolates and following sequence analysis was shown to be similar to sequences recently reported in Salmonella enterica Hadar and on an 85 kb plasmid conferring quinolone resistance in Escherichia coli.

Conclusions: Aminoglycoside aadA2-encoding class 1 integrons were identified among unrelated Campylobacter spp. Amino acid sequence comparisons revealed identical structures in both Salmonella and E. coli. The presence of class 1 integrons in Campylobacter spp. may be significant should these organisms enter the food chain and especially when antimicrobial treatment for severe infections is being considered.

Keywords: Keywords: antimicrobial resistance, Campylobacter, gene cassettes, gene organization

Journal Article.  3583 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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