Journal Article

<i>erm</i>(C) is the predominant genetic determinant for the expression of resistance to macrolides among methicillin-resistant <i>Staphylococcus aureus</i> clinical isolates in Greece

I. Spiliopoulou, E. Petinaki, P. Papandreou and G. Dimitracopoulos

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 53, issue 5, pages 814-817
Published in print May 2004 | ISSN: 0305-7453
Published online May 2004 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkh197
erm(C) is the predominant genetic determinant for the expression of resistance to macrolides among methicillin-resistant Staphylococcus aureus clinical isolates in Greece

More Like This

Show all results sharing these subjects:

  • Medical Oncology
  • Critical Care

GO

Show Summary Details

Preview

Objectives: Macrolide, lincosamide and streptogramin type B (MLSB) resistance was determined in Staphylococcus aureus clinical isolates from two University Hospitals.

Methods: Antibiotic resistance was investigated by double disc diffusion and MIC determination. Resistance determinants were detected by PCR and DNA hybridization, while clonal types were identified by pulsed-field gel electrophoresis (PFGE) analysis of SmaI DNA fragments.

Results: Among methicillin-susceptible S. aureus (MSSA) isolates, inducible and MS phenotypes were detected, with the predominance of the erm(A) gene, followed by the msr(A) and erm(C) genes. The majority of methicillin-resistant S. aureus (MRSA) isolates expressed the constitutive phenotype and carried the erm(C) gene. PFGE revealed the dissemination of two major clones among the MRSA in both hospitals.

Conclusions: erm(C) is the predominant genetic determinant for the expression of MLSB resistance among S. aureus isolates, especially MRSA, in Greece. This is due to the spread of two major clones in the country.

Keywords: Keywords: erythromycin, clindamycin, staphylococci, mechanisms, typing

Journal Article.  2453 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.