Journal Article

New aminoglycoside acetyltransferase gene, <i>aac(3)-Id</i>, in a class 1 integron from a multiresistant strain of <i>Vibrio fluvialis</i> isolated from an infant aged 6 months

Ashraf M. Ahmed, Tomoko Nakagawa, Eiji Arakawa, Thandavarayan Ramamurthy, Sumio Shinoda and Tadashi Shimamoto

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 53, issue 6, pages 947-951
Published in print June 2004 | ISSN: 0305-7453
Published online June 2004 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkh221
New aminoglycoside acetyltransferase gene, aac(3)-Id, in a  class 1 integron from a multiresistant strain of Vibrio fluvialis  isolated from an infant aged 6 months

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Objectives: To characterize the molecular basis of antibiotic resistance in a multidrug-resistant clinical isolate of Vibrio fluvialis H-08942.

Patient and methods: V. fluvialis H-08942 was isolated from a hospitalized infant aged 6 months suffering from cholera-like diarrhoea in India in 2002. The broth microdilution method was used to determine the MICs of a range of antibiotics for this strain. PCR, DNA sequencing, Southern hybridization, cloning and expression were used to characterize the molecular basis of antibiotic resistances.

Results: V. fluvialis H-08942 showed resistance to chloramphenicol, streptomycin, spectinomycin, co-trimoxazole, ampicillin, furazolidone, nalidixic acid and gentamicin. A class 1 integron that contains a novel aminoglycoside acetyltransferase gene, aac(3)-Id, and aminoglycoside adenyltransferase gene, aadA7, was characterized. The aac(3)-Id gene product was found to share 50%, 45% and 44% identity to AAC(3)-Ic, AAC(3)-Ia, and AAC(3)-Ib, respectively. Both aac(3)-Id and aadA7 genes were cloned and expressed in Escherichia coli. Phylogenetic analysis suggested that the aac(3)-Id represents a fourth evolutionary lineage in the aminoglycoside acetyltransferase genes. Southern hybridization showed that this integron is located in the chromosome.

Conclusions: In this study we identified a new type of aminoglycoside acetyltransferase gene, aac(3)-Id. In addition, this is the first report of identification of antibiotic resistance genes and a class 1 integron in V. fluvialis.

Keywords: Keywords: gentamicin resistance, aminoglycoside adenyltransferase, aadA7, cholera-like diarrhoea

Journal Article.  2867 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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