Journal Article

Rapid alternative methods for detection of rifampicin resistance in <i>Mycobacterium tuberculosis</i>

Dihadenys Lemus, Anandi Martin, Ernesto Montoro, Françoise Portaels and Juan Carlos Palomino

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 54, issue 1, pages 130-133
Published in print July 2004 | ISSN: 0305-7453
Published online July 2004 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkh320
Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis

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Objective: To study the performance of three rapid low cost methods for the detection of rifampicin resistance.

Methods: A panel of 20 coded Mycobacterium tuberculosis strains was tested blindly by the low cost methods: nitrate reductase, MTT and resazurin assays, and compared with the results obtained with the gold standard methods: the proportion method on Löwenstein–Jensen medium and the BACTEC TB 460 system. We have also tested two commercial tests: MGIT and INNO LiPA Rif.TB kit.

Results: Complete agreement was observed among all methods.

Conclusion: These three simple methods might become inexpensive alternative procedures for rapid detection of rifampicin resistance in low-resource countries.

Keywords: M. tuberculosis; MTT; resazurin; nitrate reductase assay; rifampicin

Journal Article.  3200 words. 

Subjects: Medical Oncology ; Critical Care

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