Journal Article

Tolerance of <i>Pseudomonas aeruginosa</i> to <i>Melaleuca alternifolia</i> (tea tree) oil is associated with the outer membrane and energy-dependent cellular processes

Chelsea J. Longbottom, Christine F. Carson, Katherine A. Hammer, Brian J. Mee and Thomas V. Riley

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 54, issue 2, pages 386-392
Published in print August 2004 | ISSN: 0305-7453
Published online August 2004 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkh359
Tolerance of Pseudomonas aeruginosa to Melaleuca alternifolia (tea tree) oil is associated with the outer membrane and energy-dependent cellular processes

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Objectives: The essential oil of Melaleuca alternifolia (tea tree oil) and its components have antimicrobial activity against a wide range of Gram-positive and Gram-negative bacteria, fungi and viruses. The mechanism(s) by which Pseudomonas aeruginosa NCTC 10662 maintains a decreased susceptibility to tea tree oil and components was investigated.

Results: Ethylene diamine tetraacetic acid enhanced the antimicrobial activity of tea tree oil and terpinen-4-ol against stationary phase P. aeruginosa while polymyxin B nonapeptide enhanced the activity of tea tree oil and γ-terpinene. Pre-treatment with the protonophore carbonyl cyanide m-chlorophenylhydrazone increased the susceptibility of exponential phase cells to sub-inhibitory concentrations of tea tree oil, terpinen-4-ol and γ-terpinene, indicating that intrinsic tolerance to tea tree oil and components is substantially energy dependent.

Conclusions: Increased tolerance to tea tree oil in P. aeruginosa is directly related to the barrier and energy functions of the outer membrane, and may involve efflux systems.

Keywords: terpenes; efflux; PMBN; EDTA; CCCP

Journal Article.  4225 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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