Journal Article

<i>Enterococcus faecium</i> N03-0072 carries a new VanD-type vancomycin resistance determinant: characterization of the VanD5 operon

David A. Boyd, Pamela Kibsey, Diane Roscoe and Michael R. Mulvey

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 54, issue 3, pages 680-683
Published in print September 2004 | ISSN: 0305-7453
Published online September 2004 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkh391
Enterococcus faecium N03-0072 carries a new VanD-type vancomycin resistance determinant: characterization of the VanD5 operon

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Objectives: To genotypically characterize the vancomycin resistance mechanism of Enterococcus faecium N03-0072, which was negative by PCR for the currently known van genotypes.

Methods: PCR was used to amplify the entire vancomycin resistance operon and the complete nucleotide sequence was determined by dideoxy cycle sequencing.

Results: Analysis revealed a VanD-type operon with 94% nucleotide identity to the VanD4 operon and 90% nucleotide identity to the VanD1/D3 operons. A set of universal primers was designed in order to identify all current vanD variants by PCR.

Conclusions: E. faecium N03-0072 carries a new VanD-type operon, designated VanD5.

Keywords: glycopeptide resistance; van PCR; E. faecium

Journal Article.  2313 words. 

Subjects: Medical Oncology ; Critical Care

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