Journal Article

A rapid increase in macrolide resistance in <i>Streptococcus pyogenes</i> isolated in Poland during 1996–2002

Katarzyna Szczypa, Ewa Sadowy, Radosław Izdebski and Waleria Hryniewicz

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 54, issue 4, pages 828-831
Published in print October 2004 | ISSN: 0305-7453
Published online October 2004 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkh420
A rapid increase in macrolide resistance in Streptococcus pyogenes isolated in Poland during 1996–2002

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Objectives: The aim of this study was to investigate Polish clinical isolates of Streptococcus pyogenes collected during a 7 year period using phenotypic and genotypic techniques.

Methods: A total of 816 isolates of S. pyogenes recovered from 33 medical centres in Poland were tested for their susceptibility to various antimicrobial agents. Erythromycin-resistant isolates were analysed by PFGE, multilocus sequence typing and emm typing methods.

Results: The tetracycline resistance rate was high (43%) among all S. pyogenes strains. Ninety-eight (12%) isolates were resistant to erythromycin. A low prevalence of the M phenotype (5.1%) associated with the presence of the mef(A) gene was found. All the isolates of the iMLSB phenotype harboured the erm(TR) gene. Out of the cMLSB isolates, 71.4% and 28.6% carried erm(TR) and erm(B), respectively. All isolates with erm(B) were resistant to telithromycin. PFGE analysis discerned 13 different patterns, A–N, with two predominant PFGE profiles—A (41 isolates) and B (25 isolates)—that in multilocus sequence typing corresponded, respectively, to a novel sequence type (ST) 367 and ST63. Overall, the representatives of these clones accounted for >90% of isolates of the iMLSB phenotype.

Conclusions: A significant increase in erythromycin resistance was observed among clinical S. pyogenes collected in Poland over a 7 year period driven by the spread of two epidemic clones.

Keywords: S. pyogenes; erythromycin resistance; phenotypes; genotypes

Journal Article.  1985 words. 

Subjects: Medical Oncology ; Critical Care

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