Journal Article

<i>In vitro</i> bactericidal activity of antimicrobial agents against enterohaemorrhagic <i>Escherichia coli</i>

Shigeharu Oie, Junko Ishitobi, Akihiro Sawa, Masaaki Tomita and Akira Kamiya

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 54, issue 5, pages 897-903
Published in print November 2004 | ISSN: 0305-7453
Published online November 2004 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkh442
In vitro bactericidal activity of antimicrobial agents against enterohaemorrhagic Escherichia coli

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Objectives: In vitro bactericidal activity of four antimicrobial agents was determined against nine strains of enterohaemorrhagic Escherichia coli.

Methods: Pulsed-field gel electrophoresis was carried out with the Bio-Rad Gene Path system. Each antimicrobial agent was added to logarithmic phase of enterohaemorrhagic E. coli (four strains of E. coli O157:H7, two of E. coli O26, two of E. coli O111, and one of E. coli O165) in broth to obtain a concentration of 10 or 50 mg/L, and viable cells were counted after 1, 2, 6 and 24 h.

Results: All nine strains were confirmed to differ in their DNA pattern by pulsed-field gel electrophoresis. Norfloxacin at concentrations of 10 and 50 mg/L had bactericidal effects on all nine strains of enterohaemorrhagic E. coli. However, cefoperazone, kanamycin and fosfomycin had no bactericidal effects on some strains. In particular, after addition of 10 mg/L fosfomycin or kanamycin, four of the nine strains showed proliferation.

Conclusions: Norfloxacin had marked bactericidal effects on enterohaemorrhagic E. coli. This information could be of value in planning randomized clinical trials of antimicrobial agents as treatment for enterohaemorrhagic E. coli infection.

Keywords: killing kinetics; norfloxacin; cefoperazone; kanamycin; fosfomycin

Journal Article.  2478 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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