Journal Article

Identification of a chromosome-borne class C β-lactamase from <i>Erwinia rhapontici</i>

Thierry Naas, Daniel Aubert, Sophie Vimont and Patrice Nordmann

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 54, issue 5, pages 932-935
Published in print November 2004 | ISSN: 0305-7453
Published online November 2004 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkh446
Identification of a chromosome-borne class C β-lactamase from Erwinia rhapontici

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Objectives: To characterize the β-lactamase gene content of Erwinia rhapontici.

Methods: The β-lactamase gene was cloned, sequenced and expressed in Escherichia coli.

Results: The cloned gene conferred a resistance pattern of an Ambler class C β-lactamase in E. coli. The AmpC-type enzyme had a pI value of 8.6 and shared 62% amino acid sequence identity with that of Escherichia fergusonii. The ampC gene was associated with a regulatory ampR gene and β-lactamase production was inducible.

Conclusions: This work provides further evidence of the molecular heterogeneity of β-lactamases in Erwinia spp. and that plant-pathogenic enterobacterial species may constitute a reservoir of antibiotic resistance genes.

Keywords: AmpC; inducible; cephalosporinases; E. rhapontici

Journal Article.  2185 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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